| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 高醫大學 | 2004陳思安 | 04:24.93 | 50M:29.86, 100M:1:02.27, 150M:1:35.58, 200M:2:09.52, 250M:2:43.46, 300M:3:17.66, 350M:3:51.70, 400M:4:24.93 | q01 | |
| 臺灣科大 | 2058王伊伶 | 04:29.40 | 50M:29.90, 100M:1:02.30, 150M:1:35.81, 200M:2:10.12, 250M:2:44.54, 300M:3:19.56, 350M:3:54.45, 400M:4:29.40 | q02 | |
| 北市大學 | 2104何亞璇 | 04:35.89 | 50M:30.18, 100M:1:02.74, 150M:1:36.78, 200M:2:11.06, 250M:2:46.09, 300M:3:21.93, 350M:3:58.52, 400M:4:35.89 | q03 | |
| 高雄師大 | 2028王如庭 | 04:37.41 | 50M:31.51, 100M:1:05.36, 150M:1:40.10, 200M:2:14.74, 250M:2:50.06, 300M:3:25.64, 350M:4:01.54, 400M:4:37.41 | q04 | |
| 中正大學 | 2010郭芮安 | 04:53.82 | 50M:31.66, 100M:1:07.33, 150M:1:44.69, 200M:2:22.57, 250M:3:00.43, 300M:3:38.16, 350M:4:16.20, 400M:4:53.82 | q05 | |
| 成功大學 | 2013吳依瑄 | 04:58.46 | 50M:31.25, 100M:1:05.51, 150M:1:42.50, 200M:2:21.03, 250M:3:00.47, 300M:3:39.93, 350M:4:20.22, 400M:4:58.46 | q06 | |
| 輔仁大學 | 2110呂芝妍 | | 50M:34.87, 100M:1:14.80, 150M:1:58.33, 200M:2:43.40, 250M:3:29.04, 300M:4:15.51, 350M:5:02.71, 400M:5:48.64 | | 逾時 |
| 文化大學 | 2002張若庭 | | | | 請假 |