| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 北市大學 | 范振惟,洪潔瑜,潘品蓁,謝承晉 | 03:52.47 | 50M:24.77, 100M:52.21, 150M:1:23.22, 200M:1:56.66, 250M:2:26.55, 300M:3:00.17, 350M:3:25.30, 400M:3:52.47 | q01 | |
| 臺灣大學 | 蔡睿紘,蔡逸杰,吳以恩,李怡諄 | 03:53.05 | 50M:24.48, 100M:52.51, 150M:1:17.24, 200M:1:44.85, 250M:2:14.11, 300M:2:47.11, 350M:3:18.35, 400M:3:53.05 | q02 | |
| 成功大學 | 陳柏廷,李姵萱,吳依瑄,王昱翔 | 03:53.09 | 50M:26.14, 100M:55.78, 150M:1:23.97, 200M:1:56.04, 250M:2:26.40, 300M:2:59.48, 350M:3:25.00, 400M:3:53.09 | q03 | |
| 臺灣體大 | 張啓宏,賴志豪,葉 甯,王天樂 | 03:54.67 | 50M:26.46, 100M:54.90, 150M:1:20.24, 200M:1:48.31, 250M:2:17.19, 300M:2:49.96, 350M:3:19.45, 400M:3:54.67 | q04 | |
| 臺灣師大 | 康鈞愷,黃奕誠,鄭雲禎,成喜宣 | 03:57.72 | 50M:25.45, 100M:52.79, 150M:1:19.01, 200M:1:48.91, 250M:2:18.32, 300M:2:53.02, 350M:3:24.14, 400M:3:57.72 | q05 | |
| 臺灣科大 | 呂治慧,侯柏宇,王怡臻,柯建丞 | 03:58.73 | 50M:31.63, 100M:1:05.73, 150M:1:31.89, 200M:2:00.64, 250M:2:31.18, 300M:3:04.73, 350M:3:30.33, 400M:3:58.73 | q06 | (第一棒:右肩肌貼) |
| 陽明交大 | 余定綸,謝沂芹,陳瑀婕,鍾魏永杰 | 03:58.76 | 50M:26.71, 100M:56.60, 150M:1:26.43, 200M:1:59.08, 250M:2:29.08, 300M:3:02.37, 350M:3:29.47, 400M:3:58.76 | q07 | |
| 清華大學 | 藍家騏,黃沛儀,張妃婷,戴翊安 | 04:00.56 | 50M:27.10, 100M:56.17, 150M:1:27.01, 200M:2:01.34, 250M:2:32.79, 300M:3:06.90, 350M:3:32.12, 400M:4:00.56 | q08 | |
| 海洋大學 | 姚羽宸,李羽甄,蘇柏綸,張浩瀚 | 04:00.72 | 50M:30.40, 100M:1:04.35, 150M:1:35.65, 200M:2:11.01, 250M:2:36.87, 300M:3:06.16, 350M:3:31.48, 400M:4:00.72 | | |
| 輔仁大學 | 陳立宇,許晴雯,張家瑜,蘇上紘 | 04:03.43 | 50M:26.49, 100M:56.37, 150M:1:27.06, 200M:2:02.73, 250M:2:33.16, 300M:3:07.21, 350M:3:33.37, 400M:4:03.43 | | |
| 高雄師大 | 田鎮睿,江 玟,蕭莘蓉,牟善竹 | 04:03.47 | 50M:26.27, 100M:55.61, 150M:1:26.35, 200M:2:00.36, 250M:2:32.62, 300M:3:07.53, 350M:3:33.94, 400M:4:03.47 | | |
| 國立體大 | 曾奕寧,羅玟惠,蔡心瑜,楊皓予 | 04:07.51 | 50M:27.39, 100M:57.39, 150M:1:26.78, 200M:2:00.75, 250M:2:33.99, 300M:3:09.65, 350M:3:37.21, 400M:4:07.51 | | |
| 虎尾科大 | 陳科均,蔡宸恩,邱羿甯,吳科緯 | 04:08.56 | 50M:27.88, 100M:58.23, 150M:1:27.44, 200M:2:00.72, 250M:2:33.13, 300M:3:10.61, 350M:3:37.81, 400M:4:08.56 | | |
| 中山大學 | 蕭弘亞,王璽鈞,黃琦穎,盧秩綱 | 04:17.12 | 50M:27.41, 100M:57.04, 150M:1:29.31, 200M:2:06.86, 250M:2:40.55, 300M:3:20.25, 350M:3:47.48, 400M:4:17.12 | | |
| 逢甲大學 | 呂紹瑄,陳苡溱,林宸禾,許珈銘 | 04:22.09 | 50M:34.38, 100M:1:17.75, 150M:1:50.88, 200M:2:30.22, 250M:2:56.63, 300M:3:25.89, 350M:3:52.57, 400M:4:22.09 | | |
| 嘉義大學 | 楊佩珊,劉垣霆,吳俊儒,劉芷嫺 | 04:24.86 | 50M:32.49, 100M:1:11.13, 150M:1:38.61, 200M:2:10.22, 250M:2:41.80, 300M:3:17.72, 350M:3:49.70, 400M:4:24.86 | | |