| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 成功大學 | 李姵萱,吳祐嘉,王昱翔,吳依瑄 | 04:12.96 | 50M:32.31, 100M:1:08.51, 150M:1:38.23, 200M:2:12.34, 250M:2:38.64, 300M:3:09.95, 350M:3:40.09, 400M:4:12.96 | q01 | |
| 臺灣體大 | 楊郁萱,陳義翔,王學冠,陳俞蓉 | 04:13.32 | 50M:33.71, 100M:1:09.86, 150M:1:39.65, 200M:2:15.12, 250M:2:41.41, 300M:3:11.31, 350M:3:40.66, 400M:4:13.32 | q02 | |
| 北市大學 | 吳亮昀,洪潔瑜,莊沐倫,劉姵吟 | 04:18.16 | 50M:29.01, 100M:1:00.12, 150M:1:36.13, 200M:2:16.50, 250M:2:43.05, 300M:3:14.79, 350M:3:44.29, 400M:4:18.16 | q03 | |
| 高雄師大 | 王如庭,羅楷穎,牟善竹,葉俞秀 | 04:21.32 | 50M:32.97, 100M:1:09.24, 150M:1:40.04, 200M:2:15.26, 250M:2:42.62, 300M:3:16.43, 350M:3:47.47, 400M:4:21.32 | q04 | (葉俞秀右肩、雙膝受傷肌貼) |
| 臺灣大學 | 陳思綺,李怡諄,蔡逸杰,呂宜憲 | 04:22.01 | 50M:32.60, 100M:1:08.75, 150M:1:44.61, 200M:2:26.80, 250M:2:52.62, 300M:3:24.35, 350M:3:51.86, 400M:4:22.01 | q05 | |
| 海洋大學 | 李天曦,陳岫彣,張浩瀚,姚羽宸 | 04:23.62 | 50M:30.09, 100M:1:03.42, 150M:1:39.67, 200M:2:21.97, 250M:2:48.92, 300M:3:21.40, 350M:3:50.87, 400M:4:23.62 | q06 | |
| 臺灣科大 | 王怡臻,柯建丞,侯柏宇,簡瑋伶 | 04:24.29 | 50M:35.29, 100M:1:12.96, 150M:1:43.70, 200M:2:19.01, 250M:2:45.90, 300M:3:18.29, 350M:3:49.12, 400M:4:24.29 | q07 | |
| 臺灣師大 | 成喜宣,謝沛家,康鈞愷,黃奕誠 | 04:25.23 | 50M:34.73, 100M:1:13.78, 150M:1:51.74, 200M:2:35.18, 250M:3:00.28, 300M:3:30.23, 350M:3:56.04, 400M:4:25.23 | q08 | |
| 清華大學 | 李雨儒,黃沛儀,戴翊安,藍家騏 | 04:26.94 | 50M:32.04, 100M:1:09.44, 150M:1:47.93, 200M:2:31.07, 250M:2:57.91, 300M:3:30.62, 350M:3:58.17, 400M:4:26.94 | | |
| 陽明交大 | 朱莉穎,方淳浲,余定綸,陳瑀婕 | 04:27.92 | 50M:35.93, 100M:1:16.09, 150M:1:48.21, 200M:2:26.35, 250M:2:53.37, 300M:3:24.49, 350M:3:54.83, 400M:4:27.92 | | |
| 國立體大 | 羅玟惠,方 廷,徐傳恩,蔡心瑜 | 04:30.32 | 50M:33.52, 100M:1:11.92, 150M:1:45.09, 200M:2:27.12, 250M:2:52.44, 300M:3:22.60, 350M:3:54.49, 400M:4:30.32 | | |
| 輔仁大學 | 艾晴天,李宗儒,許晴雯,陳立宇 | 04:34.49 | 50M:34.66, 100M:1:13.53, 150M:1:45.53, 200M:2:24.06, 250M:2:57.58, 300M:3:38.77, 350M:4:04.91, 400M:4:34.49 | | |
| 虎尾科大 | 蔡宸恩,陳科均,吳科緯,邱羿甯 | 04:38.28 | 50M:35.60, 100M:1:15.47, 150M:1:48.72, 200M:2:27.65, 250M:2:56.58, 300M:3:30.22, 350M:4:01.39, 400M:4:38.28 | | |
| 逢甲大學 | 陳苡溱,許珈銘,王雋升,呂紹瑄 | 04:43.61 | 50M:35.24, 100M:1:15.30, 150M:1:46.16, 200M:2:23.27, 250M:2:52.37, 300M:3:26.96, 350M:4:01.46, 400M:4:43.61 | | |
| 中山大學 | 楊翔聿,盧秩綱,王璽鈞,王亭立 | 04:47.91 | 50M:30.69, 100M:1:05.06, 150M:1:40.79, 200M:2:21.68, 250M:2:55.55, 300M:3:42.51, 350M:4:12.44, 400M:4:47.91 | | |
| 嘉義大學 | 楊佩珊,吳俊儒,劉垣霆,劉芷嫺 | 05:02.73 | 50M:38.69, 100M:1:23.15, 150M:2:01.70, 200M:2:47.95, , 300M:3:55.85, 350M:4:27.84, 400M:5:02.73 | | |