| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 清華大學 | 許修睿,魏允澄,周瑋民,林 寬 | 03:51.70 | 50M:27.97, 100M:59.37, 150M:1:26.93, 200M:1:57.47, 250M:2:24.34, 300M:2:53.77, 350M:3:21.58, 400M:3:51.70 | q01 | |
| 雲林科大 | 葉致顯,呂修誼,游智閎,鄭宇宸 | 03:51.79 | 50M:26.70, 100M:58.01, 150M:1:26.52, 200M:1:58.47, 250M:2:25.25, 300M:2:56.72, 350M:3:22.02, 400M:3:51.79 | q02 | |
| 臺灣大學 | 吳羽峻,孫郁傑,陳和遠,温柏翔 | 03:51.95 | 50M:26.82, 100M:56.73, 150M:1:24.28, 200M:1:56.43, 250M:2:23.04, 300M:2:54.33, 350M:3:21.22, 400M:3:51.95 | q03 | |
| 輔仁大學 | 臧 翰,羅仕軒,陳琮尹,王宇宸 | 03:53.82 | 50M:28.34, 100M:59.74, 150M:1:27.63, 200M:1:58.14, 250M:2:25.67, 300M:2:55.62, 350M:3:23.03, 400M:3:53.82 | q04 | |
| 陽明交大 | 胡享博,柯王翔,石柏昱,游鈞皓 | 03:54.66 | 50M:28.14, 100M:59.34, 150M:1:27.40, 200M:1:59.08, 250M:2:26.39, 300M:2:55.55, 350M:3:23.66, 400M:3:54.66 | q05 | |
| 成功大學 | 凃明旻,蕭丞佐,盧立澤,林承佑 | 03:55.48 | 50M:27.65, 100M:58.65, 150M:1:26.84, 200M:2:00.04, 250M:2:28.07, 300M:2:58.92, 350M:3:25.85, 400M:3:55.48 | q06 | |
| 政治大學 | 柯泓邦,吳岱融,黃柏淵,陳為政 | 03:56.77 | 50M:27.00, 100M:59.47, 150M:1:27.10, 200M:1:59.22, 250M:2:27.47, 300M:2:58.88, 350M:3:25.54, 400M:3:56.77 | q07 | |
| 臺北科大 | 李冠甫,游晨顥,林昱承,賴永誠 | 03:57.74 | 50M:29.37, 100M:1:00.56, 150M:1:27.89, 200M:1:57.25, 250M:2:25.94, 300M:2:58.85, 350M:3:27.17, 400M:3:57.74 | q08 | |
| 台北海大 | 曾趙又霆,林泰宏,楊之奎,曾趙子勛 | 03:58.75 | 50M:28.47, 100M:57.88, 150M:1:28.36, 200M:2:02.43, 250M:2:30.15, 300M:3:00.99, 350M:3:28.82, 400M:3:58.75 | | |
| 東華大學 | 謝麥克,李康丞,莊子毅,林哲丞 | 03:59.29 | 50M:29.09, 100M:59.07, 150M:1:28.44, 200M:2:00.95, 250M:2:29.24, 300M:2:59.99, 350M:3:29.05, 400M:3:59.29 | | |
| 東海大學 | 三島遙仁,許晉愷,陳 紘,王俊富 | 03:59.60 | 50M:28.04, 100M:59.09, 150M:1:29.15, 200M:2:01.11, 250M:2:29.78, 300M:3:01.77, 350M:3:27.85, 400M:3:59.60 | | |
| 高雄科大 | 黃嘉笙,張晏鑫,朱宸宥,李侑勳 | 03:59.83 | 50M:28.82, 100M:59.09, 150M:1:26.36, 200M:1:57.38, 250M:2:25.71, 300M:3:00.01, 350M:3:27.62, 400M:3:59.83 | | (李：左手腕骨折肌貼) |
| 臺灣師大 | 李昭奕,蔡承佑,何庭宇,李睿濱 | 04:00.35 | 50M:28.29, 100M:1:00.65, 150M:1:28.93, 200M:2:01.12, 250M:2:28.30, 300M:3:01.03, 350M:3:28.90, 400M:4:00.35 | | |
| 淡江大學 | 賴績智,張育晨,周庭安,蔡易呈 | 04:00.48 | 50M:29.23, 100M:1:00.16, 150M:1:27.97, 200M:2:00.33, 250M:2:29.29, 300M:3:01.98, 350M:3:29.51, 400M:4:00.48 | | |
| 中山大學 | 楊承域,黃崧原,莊印哲,李孟炫 | 04:04.71 | 50M:29.97, 100M:1:05.35, 150M:1:33.15, 200M:2:05.67, 250M:2:33.56, 300M:3:04.30, 350M:3:31.44, 400M:4:04.71 | | |
| 臺北醫大 | 賴宥安,黃彥綸,程威綸,邱泰維 | 04:10.73 | 50M:29.36, 100M:1:01.05, 150M:1:30.16, 200M:2:02.36, 250M:2:32.39, 300M:3:07.26, 350M:3:37.44, 400M:4:10.73 | | |
| 臺灣科大 | 卜鄭謙,劉豐宇,洪祥赫,彭琨泰 | 04:12.26 | 50M:29.77, 100M:1:03.22, 150M:1:31.63, 200M:2:07.13, 250M:2:35.49, 300M:3:05.64, 350M:3:36.07, 400M:4:12.26 | | |
| 嘉義大學 | 李重恩,王傳鈞,吳紹廷,孫翊凱 | 04:13.63 | 50M:30.66, 100M:1:08.85, 150M:1:37.90, 200M:2:11.56, 250M:2:39.19, 300M:3:09.30, 350M:3:39.39, 400M:4:13.63 | | |
| 中央大學 | 葉柏劭,林宥安,陳孝傑,黃少楷 | | 50M:32.12, 100M:1:06.96, 150M:1:36.96, 200M:2:13.97, 250M:2:43.55, 300M:3:18.09, 350M:3:51.52, 400M:4:36.05 | | 逾時 |
| 靜宜大學 | 張証崴,李宗儒,卓傑元,施程瀚 | | 50M:33.74, 100M:1:10.58, 150M:1:42.48, 200M:2:19.20, 250M:2:49.58, 300M:3:24.96, 350M:3:54.36, 400M:4:34.06 | | 逾時 |
| 逢甲大學 | 廖 為,葉書亞,徐瑀優,洪譽境 | | 50M:30.43, 100M:1:05.45, 150M:1:37.49, 200M:2:13.44, 250M:2:50.19, 300M:3:33.09, 350M:4:01.30, 400M:4:33.02 | | 逾時 |
| 國防醫大 | 劉秉祐,許熙賢,張簡子祈,徐皓恩 | | 50M:30.76, 100M:1:05.61, 150M:1:38.34, 200M:2:16.45, 250M:2:50.30, 300M:3:28.34, 350M:3:59.64, | | 逾時 |
| 元智大學 | | | | | 請假 |